Hanina Hibshoosh, MD
Faculty Director of MPSR
Dept. of Pathology & Cell Biology
Title: Overview of of Molecular Pathology Shared Resource (MPSR)
Description: The Molecular Pathology Shared Resource (MPSR) of The Herbert Irving Comprehensive Cancer Center (HICCC) of Columbia University (MPSR) facilitates tissue-based research by the acquisition, processing, and characterization of tissues, providing related data, materials, and diverse expertise that enables discovery, characterization, and validation via three integrated services: (1) Routine tissue banking, patient/project/trial specific banking, and Next Generation Banking (characterizing captured frozen tissue) and FFPE procurement; (2) Processing: End to end histology services, TMA construction, immunohistochemistry/IF service, DNA/RNA extraction, slide image digitization service; (3) Characterization: H&E and IHC/IF slide review, image analysis, RNA/DNA quantification and quality assessment. The former is directed by Dr Hanina Hibshoosh. By the integration of the Human Immune Monitoring Core, directed by Dr Benjamin Izar, to the MPSR this past year we dramatically expanded our characterization capabilities with state of the art genomics and multiplex tissue imaging via:single cell RNA sequencing with 10XGenomics (10Xchromium controller and X) platform, Spatial transcriptomic sequencing with 10X Visium (including Visium HD), Spatial Molecular Imaging Nanostring CosMX platform, Rapid high parametric RNA analysis with Nanostring nCounter system, and multiplex immune fluorescence for tissue analysis with Akoya PhenoImager.
We currently service more than 379 unique investigators annually and fulfill more than 440K granular service requests, 4500 bundled service requests and support >157 IRBs at Columbia University.
Benjamin Izar, MD, PhD
Faculty Director of HIMC
Dept. of Med Hematology and Oncology
Title: Overview of Human Immune Monitoring Core (HIMC)
Description: Benjamin Izar, MD, Faculty Director of Human Immune Monitoring Core (HIMC) will share an overview of HIMC. The Human Immune Monitoring Core (HIMC) offers high-quality, cost-effective services for in-depth, multi-omics immune profiling and monitoring at single-cell resolution, powered by state-of-the-art technologies and a skilled team of physicians, scientists, and technicians. Driven by innovation and project-focused, HIMC utilizes the latest advancements in immunology to develop robust assays and optimized methods. HIMC provides comprehensive support from experimental design and sample processing to data acquisition, analysis, and management.
Zoom Meeting
https://columbiacuimc.zoom.us/j/97847371419?pwd=hNTcbh48XWXbHY2BMFflr6wLYQonOo.1
Meeting ID: 978 4737 1419
Passcode: seminar