(Pancreas tissue processing and Islet purification/quality control at CCTI- the Columbia Center for Translational Immunology)
Human and large animal islet isolation
The CCTI Islet Core is a campus-based lab that is committed to providing the highest quality human and large animal islets to support the local diabetes research community. Expert personnel familiar with clinical islet transplantation will perform the technically challenging isolation of islets and can predictably provide islets that are high in quantity, quality and purity. The Islet Core also offers crude digests or non-islet cells of pancreatic tissue from deceased human pancreas donors, non-human primates, and pigs for research. The Core will coordinate and provide islets to investigators when research need matches the availability of a human research pancreas or a prearranged large animal islet experiment.
The Core also provides training and expert advice in isolation and culture of pancreatic islets, as well as ex vivo analyses of islet cell viability, function, and islet equivalence calculation. Moreover, the Core can provide advice on streptozotocin induction of diabetes and transplantation of islets in mice.
The Core offers training in:
- Human and mouse islet isolation
- Islet culture techniques
- In vitro islet glucose challenge using the static incubation approach
- Islet viability testing (FDA/PI)
- Determining islet insulin, glucagon, and DNA content
- Sub-renal capsular or intra-portal Islet transplantation in mice
- Graft retrieval for morphology and other determinations
The philosophy of the Islet Core is to provide access to large animal and human research islets in a highly efficient and economical fashion. Recharge rates are reviewed and approved by the Columbia Recharge Office. Rates are available by contacting Dr. Xiaojuan Chen.